Jump into 3D Organoids: Basic Culture Techniques and Advanced Applications

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Mel Robbins: The Let Them Theory, Build a Business and Life on Your Terms | Human Behavior | E329 1:16:55

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On her son’s prom night, Mel Robbins fussed over details that didn’t matter. Her daughter grabbed her arm and said, “Let them. Let them run in the rain. Let them eat where they want. Let them.” Those two simple words hit Mel like a ton of bricks and completely changed how she thinks about control. In this episode, Mel shares some of the pivotal moments that shaped her career, her innovative strategies for overcoming adversity, and how the Let Them Theory can help you navigate business challenges, strengthen relationships, and unlock your true power. In this episode, Hala and Mel will discuss: (00:00) Introduction (02:57) The Power of Action (04:22) Mel's Unforgettable TED Talk Debut (07:00) The 5 Second Rule (07:52) Building Unshakable Confidence (12:04) Turning Adversity into Strength (22:00) The Power of Showing Up for Others (30:40) Why Details Matter in Business (42:32) Understanding the Let Them Theory (51:14) The Let Them Theory in Business Mel Robbins is a motivational speaker, the host of The Mel Robbins Podcast, and a bestselling author of several influential books, including her latest, The Let Them Theory. Known for her groundbreaking 5 Second Rule, she has helped millions of people take action and transform their lives. With 30 million views, her TEDx talk made her a recognized voice in behavior change. Mel is also the CEO of 143 Studios, a female-driven media company creating award-winning content for top brands like LinkedIn and Audible. She is a Forbes 50 Over 50 Honoree and one of USA Today’s Top 5 Mindset Coaches. Resources Mentioned: Mel’s Books: The Let Them Theory: A Life-Changing Tool That Millions of People Can't Stop Talking About : https://amzn.to/4h6quLh The 5 Second Rule: Transform your Life, Work, and Confidence with Everyday Courage : https://amzn.to/3WdAgTX Sponsored By: OpenPhone - Get 20% off 6 months at https://www.openphone.com/PROFITING Shopify - Sign up for a one-dollar-per-month trial period at https://www.youngandprofiting.co/shopify Airbnb - Your home might be worth more than you think. Find out how much at https://www.airbnb.com/host Rocket Money - Cancel your unwanted subscriptions and reach your financial goals faster with Rocket Money. Go to https://www.rocketmoney.com/profiting Indeed - Get a $75 job credit at indeed.com/profiting Active Deals - youngandprofiting.com/deals Key YAP Links Reviews - ratethispodcast.com/yap Youtube - youtube.com/c/YoungandProfiting LinkedIn - linkedin.com/in/htaha/ Instagram - instagram.com/yapwithhala/ Social + Podcast Services : yapmedia.com Transcripts - youngandprofiting.com/episodes-new All Show Keywords: Entrepreneurship, entrepreneurship podcast, Business, Business podcast, Self Improvement, Self-Improvement, Personal development, Starting a business, Strategy, Investing, Sales, Selling, Psychology, Productivity, Entrepreneurs, AI, Artificial Intelligence, Technology, Marketing, Negotiation, Money, Finance, Side hustle, Startup, mental health, Career, Leadership, Mindset, Health, Growth mindset. Career, Success, Entrepreneurship, Productivity, Careers, Startup, Entrepreneurs, Business Ideas, Growth Hacks, Career Development, Money Management, Opportunities, Professionals, Workplace, Career podcast, Entrepreneurship podcast…

cách đây khoảng một năm trước 1:01:26

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Organoids are three-dimensional in vitro cultures derived directly from patient tissues or induced pluripotent stem cells (iPSC) and embryonic stem cells. They self-organize to generate structures that resemble the tissue of origin and recapitulate the key physiological functions of the parental organ.

In this episode of Listen In, explore the benefits of organoids as infectious disease models and drug screening vehicles, learn how to start organoid culture in your laboratory, and discover a portfolio of organoids for your research applications.

Watch the full presentation here: https://events.bitesizebio.com/jump-into-3d-organoids-basic/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

138 tập

#Science #Biology #Bitesize Bio #Bitesizebio #Bitesize #Bios #Webinars

Jump into 3D Organoids: Basic Culture Techniques and Advanced Applications

Listen In - Bitesize Bio Webinar Audios

published cách đây khoảng một năm trước

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MP3•Trang chủ episode

Watch the full presentation here: https://events.bitesizebio.com/jump-into-3d-organoids-basic/join

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

138 tập

#Science #Biology #Bitesize Bio #Bitesizebio #Bitesize #Bios #Webinars

Tất cả các tập

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AI-Powered 3D Organoid Analysis 1:02:09

cách đây 6 ngày trước1:02:09

1:02:09

Multicolor 3D fluorescent confocal microscopy presents significant challenges in image acquisition and data analysis. However, AI is making a massive impact on 3D spatial biology. This episode of Listen In explores the incorporation of AIVIA, an AI-powered image analysis software, into workflows to address these challenges in analyzing multicolor fluorescent microscopy data of 3D organoids. See how AIVIA’s tools enhance the visualization and quantification of cellular structures and organoids, enabling precise segmentation and analysis of fluorescent signals. And get a showcase of how AIVIA supports Python-based image analysis for microscopists. Watch the full presentation here: https://events.bitesizebio.com/ai-powered-3d-organoid-analysis Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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High-Throughput and Wide-Range Protein Concentration Determination of Monoclonal Antibodies 27:57

cách đây 6 weeks trước27:57

27:57

In this episode of Listen In, explore high-throughput and wide-range protein concentration analysis that can deliver a 20-fold improvement in time and budget savings. Protein concentration is an obligatory critical quality attribute necessary to determine total protein content in biopharmaceutical formulations. Concentration determination is necessary before many downstream assays. A UV-based spectrophotometric assay using the protein’s extinction coefficient is the most typical method. Conventional UV methods or a specialized approach using variable pathlength slope spectroscopy could take one day to measure 15 samples in triplicate. Larger batches would require multiple analysts for a single-day turnaround time. With vast studies spanning multiple projects, protein concentration analysis becomes a key deliverable before all other methods can be performed. Thus, efficient rapid concentration analysis with a minimal volume requirement is essential. Hit play to learn how the Lunatic (Unchained Labs, Pleasanton, CA) offers rapid, plate-based concentration measurement with minimal prep and sample volume requirements. It utilizes only two microliters per sample, can analyze 96 wells in less than 12 minutes, and provides accurate measurements over a broad concentration range, from 0.03 mg/mL to 176 mg/mL. Watch the full presentation here: https://events.bitesizebio.com/high-throughput-and-wide-range/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Multiplexing Magic: How MULTI-seq Revolutionizes Single-Cell RNA Sequencing 48:51

cách đây 16 weeks trước48:51

48:51

Learn more about the capabilities of MULTI-seq in single-cell sequencing in this episode of Listen In . Developed by the Gartner Lab at UCSF , this innovative multiplexing method increases sample and cell throughput while minimizing cost and improving your data quality. From modest experiments to expansive 96-plex studies and beyond, MULTI-seq enables truly mechanistic studies. Hit play to explore the potential of this tool and how it could benefit your research. Watch the full presentation here: https://events.bitesizebio.com/multiplexing-magic-how-multi-seq/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Multi-View Light Sheet Microscopy to Track Cell Lineages in Tissue Morphogenesis 56:51

cách đây 18 weeks trước56:51

56:51

A major question in developmental biology is understanding how cellular fate decisions are regulated precisely in space and time. Fortunately, we can now begin to observe these processes with modern imaging approaches, which allow the tracking of cell fate decision events and cellular rearrangements by live 3D time-lapse microscopy. This development is shifting our understanding of pattern formation in development away from static models towards models based on the principles of dynamical systems and statistical mechanics. In this episode of Listen In , hear how light-sheet microscopy is the technique of choice for volumetric live imaging of 3D samples at high speed with minimal phototoxicity. See two user examples of how light-sheet microscopy is helping scientists understand the biological process of intestinal and brain organoid development. We also explore the technology behind the Leica Viventis LS2 Live, which combines dual-view illumination and detection in an open-top configuration. You will also learn how this unique configuration allows easy sample mounting and long-term imaging from small embryos and organoids to large multicellular systems. Watch the full presentation here: https://microscopyfocus.com/multi-view-light-sheet-microscopy-to-track-cell-lineages-in-tissue-morphogenesis/ "Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Enhancing 3D Spatial Biology with AI: Simplified Insights for All 1:09:11

cách đây 20 weeks trước1:09:11

1:09:11

In this episode, learn how AI-powered segmentation, spatial analysis, and phenotyping can help you gain new insights for 3D images with complex morphological measurements featuring up to 15 biomarkers. See how to characterize tissue microenvironments and examine the differences between normal and disease tissues without the need to code or train Deep Learning models—all with Aivia. Plus, get your 3D spatial biology results faster with an inbuilt, enhanced Deep Learning model that can accurately detect and partition cells with morphological variations. You will discover how to leverage your expertise or simple automation to classify cells into different phenotypes and interactively explore them in their spatial context. We also demonstrate how, with a few clicks, you can produce key measurements such as percentage distribution, Pearson correlation coefficient, and dimensionality reduction. You will also learn how to visualize the relationship between biomarkers and clusters using dendrograms. Watch the full presentation here: https://microscopyfocus.com/enhancing-3d-spatial-biology-with-ai-simplified-insights-for-all/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Accelerate Scientific Progress: The Power of Reproducibility, Collaboration and New Imaging Technologies 1:12:31

cách đây 21 weeks trước1:12:31

1:12:31

Imaging scientists have a critical educational role in improving rigor and reproducibility by sharing their technical expertise and providing intellectual contributions in all aspects of image-based science. They participate in creating, developing, and disseminating guidelines, resources, and tools to improve image-based research. In this episode, learn what impacts reproducibility in microscopy, discover resources and initiatives for improving education, rigor, and reproducibility, and see how collaboration drives innovation. Watch the full presentation here: https://microscopyfocus.com/accelerate-scientific-progress-the-power-of-reproducibility-collaboration-and-new-imaging-technologies/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Inside Immunoassays: Best Practices for Accurate and Reliable Results 56:26

cách đây 22 weeks trước56:26

56:26

Immunoassays play an indispensable role in scientific research. They facilitate the detection and quantification of specific analytes, enabling in-depth investigations into disease mechanisms, biomarker discovery, and evaluation of the potential of therapeutic interventions. Sample selection and preparation are of utmost importance, as they have a direct impact on the accuracy, reliability, and integrity of the obtained results. Properly selecting and preparing samples ensures that they are representative of the population under study and that the analytes of interest are preserved in their native state. In addition, controlling and mitigating matrix interference is crucial in immunoassays to minimize the influence of sample components that may hinder the accuracy of measurements. In this episode, hear from immunoassay experts as they guide you through all these areas. Watch the full presentation here: https://events.bitesizebio.com/inside-immunoassays-best-practices/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Windows On Neurovascular Pathologies 1:03:18

cách đây 23 weeks trước1:03:18

1:03:18

The brain is an immune-privileged organ, meaning it is protected from the entry of pathogens, blood circulating factors, and immune cells by a physical barrier called the blood-brain barrier (BBB). But pathological events such as ischemia (stroke), trauma (traumatic brain injury), or tumor growth (gliomas and brain metastasis), activate the vascular endothelium, disturb or disrupt the BBB and allow immune cell homing. In this episode, discover how innate immunity can sustain deleterious effects following brain trauma, and the technological developments enabling longitudinal studies into post-trauma lesion remodeling. Watch the full presentation here: https://microscopyfocus.com/windows-on-neurovascular-pathologies/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Unleashing Fibrotic Secrets: Coupling the Power of Laser Microdissection with Mass Spec 45:41

cách đây 24 weeks trước45:41

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Tissue heterogeneity is a significant challenge when analyzing proteins in tissue samples to identify disease states. In this episode, explore how combining laser microdissection with mass spectrometry (LCM-MS) on fixed and stained tissues lets you explore the protein networks within pathological features to shed light on their molecular functions. Using hallmark lesions of lung fibrosis in the honeycomb airways and fibroblastic foci as an example, you'll hear how this technology can detect over 4,900 proteins from tissue volumes as low as 0.005 mm3. Watch the full presentation here: https://events.bitesizebio.com/unleashing-fibrotic-secrets/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Virtual Reality Showcase for Stellaris: Leica’s Confocal Microscopy Platform 25:07

cách đây 25 weeks trước25:07

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Join Advanced Workflow Specialists for a virtual reality showcase of Leica's confocal microscopy platform, STELLARIS. In this episode of Listen In , discover how to enhance your confocal imaging from performing multicolor experiments with clear, sharp images to capturing fluorescence intensity and lifetime-based information simultaneously. Watch the full presentation here: https://microscopyfocus.com/confocal-microscopy-stellaris-showcase/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Tools & Techniques for the Study of Biologics & Antibody Drug Conjugates 47:57

cách đây 26 weeks trước47:57

47:57

The fundamental cellular mechanisms of internalization and trafficking are crucial to many areas of cell biology, especially the proper function of therapeutic antibodies. Antibodies intended for use as antibody-drug conjugates (ADCs) should specifically bind to target cells and rapidly internalize via endocytosis. However, the ability to study these processes has historically been limited by the lack of tools that directly monitor the internalization and subsequent catabolism of extracellular material. In this episode, get a demo of rapid, high-throughput methods to screen for antibody internalization. You will also hear about techniques to directly visualize the internalization of therapeutic antibodies and ADCs in live cells for structure-activity relationship studies. You will also learn how the insights provided by these approaches have the potential to accelerate biotherapeutic lead generation and development. Watch the full presentation here: https://events.bitesizebio.com/tools-techniques-for-the-study-of/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Multiplex Annotated Tissue Imaging System (MANTIS®) 1:07:54

cách đây 27 weeks trước1:07:54

1:07:54

Join us on Listen In as we unveil a breakthrough method for imaging skin immune cells, crucial for accurate diagnoses and personalized treatments. Traditional imaging faces challenges like high tissue autofluorescence and spectral spillover. However, our latest episode introduces a game-changing solution using the Leica SP8 microscope coupled with the MANTIS system. Learn how this innovative approach allows for the acquisition of up to 10 colors, providing detailed immune phenotyping of skin samples. Plus, discover how MANTIS simplifies complex imaging, making advanced analysis accessible and practical for routine clinical practice. Tune in to explore how this new technology can revolutionize the identification of skin immune cells and enhance diagnostic accuracy. Watch the full presentation here: https://microscopyfocus.com/multiplex-annotated-tissue-imaging/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Breaking Out of The Mold - A Panel Discussion of Alternative Careers 50:20

cách đây 28 weeks trước50:20

50:20

Join us on Listen In for an inspiring episode that explores alternative careers for scientists who want to combine their artistic skills with their passion for science. If you love science but feel that traditional lab life isn’t for you, this episode is a must-listen. In this episode, we're joined by a panel of individuals who have carved out unique careers that merge science with creativity. Our panelists include a Marketing Communication Manager/Graphic Designer, an Artist in Residence, and a Science Rapper. These trailblazers will share their journeys, discuss how they found their unique paths, and provide insights into their innovative careers. Learn about the various opportunities available beyond the conventional academic and industry roles, and get inspired by the possibilities of combining science with art. Tune in to discover how you can turn your passion for science and art into a fulfilling career. Watch the full presentation here: https://events.bitesizebio.com/breaking-out-of-the-mold-a-panel-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Five-Color STED With a Single Depletion Laser and Fluorescence Lifetime Phasor Separation 56:33

cách đây 29 weeks trước56:33

56:33

Dive into the innovative world of STED microscopy and explore how researchers are pushing the boundaries of super-resolution imaging in this episode of Listen In. Traditional STED microscopy achieves high resolution by using a doughnut-shaped depletion laser to selectively suppress fluorescence, but multicolor experiments often face challenges such as laser misalignment and dye bleaching. Explore the game-changing technique of using a single depletion wavelength combined with phasor-based FLIM separation. This method allows scientists to distinguish multiple fluorophores with similar emission spectra but different fluorescent lifetimes, significantly increasing the number of usable spectral channels. Discover how researchers successfully depleted five fluorochromes across different channels using a single laser and utilized the phasor plot for quick and easy visualization of staining quality and lifetime analysis. This approach not only doubles the number of distinguishable colors in laser scanning microscopy but also simplifies the process, making it accessible to many scientists with minimal training. Watch the full presentation here: https://microscopyfocus.com/five-color-sted/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Grant Writing: Everything but the Science 32:47

cách đây 30 weeks trước32:47

32:47

Dive into the often daunting world of grant applications. Securing funding is critical for any researcher, but the process can be filled with tricky tasks and complex requirements. Join us as we explore strategies to handle common challenges in grant applications with expert advice. Learn how to tackle issues like: • Career disruption • Reason for choosing your place of work • Track record relative to opportunity • Consumer involvement This episode guides you through the rationale behind these additional requirements, offers tips on how to effectively showcase your track record and collaborators, and provides insights on managing challenging grant situations. Don’t miss this essential advice that could make all the difference in securing your next grant! Watch the full presentation here: https://events.bitesizebio.com/grant-writing-everything-but-the-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com…

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Jump into 3D Organoids: Basic Culture Techniques and Advanced Applications