New TauSTED Tools for Gentle Live Imaging at Nanoscale Resolution

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How To Replace A $100,000+ Salary Within 6 MONTHS Through Buying A Small Business w/ Alex Kamenca & Carley Mitus 57:50

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Alex (@alex_kamenca) and Carley (@carleymitus) are both members of our Action Academy Community that purchased TWO small businesses last thursday! Want To Quit Your Job In The Next 6-18 Months Through Buying Commercial Real Estate & Small Businesses? 👔🏝️ Schedule A Free 15 Minute Coaching Call With Our Team Here To Get "Unstuck" Want to know which investment strategy is best for you? Take our Free Asset-Selection Quiz Check Out Our Bestselling Book : From Passive To Passionate : How To Quit Your Job - Grow Your Wealth - And Turn Your Passions Into Profits Want A Free $100k+ Side Hustle Guide ? Follow Me As I Travel & Build: IG @brianluebben ActionAcademy.com…

cách đây khoảng một năm trước 1:04:16

MP3•Trang chủ episode

This episode of Listen In showcases the latest tools and developments for multiplex STED imaging of living specimens at nanoscale resolution.

Confocal imaging is a fundamental tool for studying the complex interplay of biomolecules, molecular machines, and higher-order cellular structures owing to its optical sectioning, sensitivity, and temporal and spatial resolution capabilities.

Imaging complex cellular structures at nanoscale resolution while characterizing the dynamics of multiple species in live specimens are emerging avenues to shed light on biological processes in their native physiological context.

With the advent of STED, researchers have realized the visualization of intracellular structures at the nanoscale. Tune in to see how STED is enabling unprecedented insights into cellular behavior, interactions, and function.

Watch the full presentation here: https://microscopyfocus.com/new-tausted-tools-for-gentle-live-imaging-at-nanoscale-resolution/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

139 tập

#Science #Biology #Bitesize Bio #Bitesizebio #Bitesize #Bios #Webinars

New TauSTED Tools for Gentle Live Imaging at Nanoscale Resolution

Listen In - Bitesize Bio Webinar Audios

published cách đây khoảng một năm trước

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MP3•Trang chủ episode

This episode of Listen In showcases the latest tools and developments for multiplex STED imaging of living specimens at nanoscale resolution.

Watch the full presentation here: https://microscopyfocus.com/new-tausted-tools-for-gentle-live-imaging-at-nanoscale-resolution/

Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/

139 tập

#Science #Biology #Bitesize Bio #Bitesizebio #Bitesize #Bios #Webinars

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Inside Immunoassays: Strategies to Maximize Your Immunoassay Data and Insights 37:24

cách đây 1 day trước37:24

37:24

Immunoassays are pivotal in scientific research, enabling the precise identification and measurement of specific analytes. They pave the way for investigations into disease mechanisms, discovering biomarkers, and evaluating the efficacy of potential therapeutic interventions. The key reagents included in an immunoassay can significantly impact reliability and reproducibility, underscoring the importance of understanding critical components within immunoassay kits. When selecting an immunoassay platform, it's essential to consider critical elements such as multiplexing capabilities and sensitivity needs as part of the experimental design. Additionally, understanding the various assay formats and their data outputs can aid in the immunoassay selection process. In this episode, immunoassay experts give practical guidance in these areas, empowering researchers to achieve more precise and meaningful results, thereby advancing knowledge and understanding in various scientific fields. MilliporeSigma is the U.S. and Canada Life Science business of Merck KGaA, Darmstadt, Germany. For Research Use Only. Not For Use In Diagnostic Procedures.…

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AI-Powered 3D Organoid Analysis 1:02:09

cách đây 29 ngày trước1:02:09

1:02:09

Multicolor 3D fluorescent confocal microscopy presents significant challenges in image acquisition and data analysis. However, AI is making a massive impact on 3D spatial biology. This episode of Listen In explores the incorporation of AIVIA, an AI-powered image analysis software, into workflows to address these challenges in analyzing multicolor fluorescent microscopy data of 3D organoids. See how AIVIA’s tools enhance the visualization and quantification of cellular structures and organoids, enabling precise segmentation and analysis of fluorescent signals. And get a showcase of how AIVIA supports Python-based image analysis for microscopists. Watch the full presentation here: https://events.bitesizebio.com/ai-powered-3d-organoid-analysis Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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High-Throughput and Wide-Range Protein Concentration Determination of Monoclonal Antibodies 27:57

cách đây 9 weeks trước27:57

27:57

In this episode of Listen In, explore high-throughput and wide-range protein concentration analysis that can deliver a 20-fold improvement in time and budget savings. Protein concentration is an obligatory critical quality attribute necessary to determine total protein content in biopharmaceutical formulations. Concentration determination is necessary before many downstream assays. A UV-based spectrophotometric assay using the protein’s extinction coefficient is the most typical method. Conventional UV methods or a specialized approach using variable pathlength slope spectroscopy could take one day to measure 15 samples in triplicate. Larger batches would require multiple analysts for a single-day turnaround time. With vast studies spanning multiple projects, protein concentration analysis becomes a key deliverable before all other methods can be performed. Thus, efficient rapid concentration analysis with a minimal volume requirement is essential. Hit play to learn how the Lunatic (Unchained Labs, Pleasanton, CA) offers rapid, plate-based concentration measurement with minimal prep and sample volume requirements. It utilizes only two microliters per sample, can analyze 96 wells in less than 12 minutes, and provides accurate measurements over a broad concentration range, from 0.03 mg/mL to 176 mg/mL. Watch the full presentation here: https://events.bitesizebio.com/high-throughput-and-wide-range/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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cách đây 20 weeks trước48:51

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Learn more about the capabilities of MULTI-seq in single-cell sequencing in this episode of Listen In . Developed by the Gartner Lab at UCSF , this innovative multiplexing method increases sample and cell throughput while minimizing cost and improving your data quality. From modest experiments to expansive 96-plex studies and beyond, MULTI-seq enables truly mechanistic studies. Hit play to explore the potential of this tool and how it could benefit your research. Watch the full presentation here: https://events.bitesizebio.com/multiplexing-magic-how-multi-seq/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Multi-View Light Sheet Microscopy to Track Cell Lineages in Tissue Morphogenesis 56:51

cách đây 21 weeks trước56:51

56:51

A major question in developmental biology is understanding how cellular fate decisions are regulated precisely in space and time. Fortunately, we can now begin to observe these processes with modern imaging approaches, which allow the tracking of cell fate decision events and cellular rearrangements by live 3D time-lapse microscopy. This development is shifting our understanding of pattern formation in development away from static models towards models based on the principles of dynamical systems and statistical mechanics. In this episode of Listen In , hear how light-sheet microscopy is the technique of choice for volumetric live imaging of 3D samples at high speed with minimal phototoxicity. See two user examples of how light-sheet microscopy is helping scientists understand the biological process of intestinal and brain organoid development. We also explore the technology behind the Leica Viventis LS2 Live, which combines dual-view illumination and detection in an open-top configuration. You will also learn how this unique configuration allows easy sample mounting and long-term imaging from small embryos and organoids to large multicellular systems. Watch the full presentation here: https://microscopyfocus.com/multi-view-light-sheet-microscopy-to-track-cell-lineages-in-tissue-morphogenesis/ "Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Enhancing 3D Spatial Biology with AI: Simplified Insights for All 1:09:11

cách đây 23 weeks trước1:09:11

1:09:11

In this episode, learn how AI-powered segmentation, spatial analysis, and phenotyping can help you gain new insights for 3D images with complex morphological measurements featuring up to 15 biomarkers. See how to characterize tissue microenvironments and examine the differences between normal and disease tissues without the need to code or train Deep Learning models—all with Aivia. Plus, get your 3D spatial biology results faster with an inbuilt, enhanced Deep Learning model that can accurately detect and partition cells with morphological variations. You will discover how to leverage your expertise or simple automation to classify cells into different phenotypes and interactively explore them in their spatial context. We also demonstrate how, with a few clicks, you can produce key measurements such as percentage distribution, Pearson correlation coefficient, and dimensionality reduction. You will also learn how to visualize the relationship between biomarkers and clusters using dendrograms. Watch the full presentation here: https://microscopyfocus.com/enhancing-3d-spatial-biology-with-ai-simplified-insights-for-all/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Accelerate Scientific Progress: The Power of Reproducibility, Collaboration and New Imaging Technologies 1:12:31

cách đây 24 weeks trước1:12:31

1:12:31

Imaging scientists have a critical educational role in improving rigor and reproducibility by sharing their technical expertise and providing intellectual contributions in all aspects of image-based science. They participate in creating, developing, and disseminating guidelines, resources, and tools to improve image-based research. In this episode, learn what impacts reproducibility in microscopy, discover resources and initiatives for improving education, rigor, and reproducibility, and see how collaboration drives innovation. Watch the full presentation here: https://microscopyfocus.com/accelerate-scientific-progress-the-power-of-reproducibility-collaboration-and-new-imaging-technologies/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Inside Immunoassays: Best Practices for Accurate and Reliable Results 56:26

cách đây 25 weeks trước56:26

56:26

Immunoassays play an indispensable role in scientific research. They facilitate the detection and quantification of specific analytes, enabling in-depth investigations into disease mechanisms, biomarker discovery, and evaluation of the potential of therapeutic interventions. Sample selection and preparation are of utmost importance, as they have a direct impact on the accuracy, reliability, and integrity of the obtained results. Properly selecting and preparing samples ensures that they are representative of the population under study and that the analytes of interest are preserved in their native state. In addition, controlling and mitigating matrix interference is crucial in immunoassays to minimize the influence of sample components that may hinder the accuracy of measurements. In this episode, hear from immunoassay experts as they guide you through all these areas. Watch the full presentation here: https://events.bitesizebio.com/inside-immunoassays-best-practices/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Windows On Neurovascular Pathologies 1:03:18

cách đây 26 weeks trước1:03:18

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The brain is an immune-privileged organ, meaning it is protected from the entry of pathogens, blood circulating factors, and immune cells by a physical barrier called the blood-brain barrier (BBB). But pathological events such as ischemia (stroke), trauma (traumatic brain injury), or tumor growth (gliomas and brain metastasis), activate the vascular endothelium, disturb or disrupt the BBB and allow immune cell homing. In this episode, discover how innate immunity can sustain deleterious effects following brain trauma, and the technological developments enabling longitudinal studies into post-trauma lesion remodeling. Watch the full presentation here: https://microscopyfocus.com/windows-on-neurovascular-pathologies/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Unleashing Fibrotic Secrets: Coupling the Power of Laser Microdissection with Mass Spec 45:41

cách đây 27 weeks trước45:41

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Tissue heterogeneity is a significant challenge when analyzing proteins in tissue samples to identify disease states. In this episode, explore how combining laser microdissection with mass spectrometry (LCM-MS) on fixed and stained tissues lets you explore the protein networks within pathological features to shed light on their molecular functions. Using hallmark lesions of lung fibrosis in the honeycomb airways and fibroblastic foci as an example, you'll hear how this technology can detect over 4,900 proteins from tissue volumes as low as 0.005 mm3. Watch the full presentation here: https://events.bitesizebio.com/unleashing-fibrotic-secrets/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Virtual Reality Showcase for Stellaris: Leica’s Confocal Microscopy Platform 25:07

cách đây 28 weeks trước25:07

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Join Advanced Workflow Specialists for a virtual reality showcase of Leica's confocal microscopy platform, STELLARIS. In this episode of Listen In , discover how to enhance your confocal imaging from performing multicolor experiments with clear, sharp images to capturing fluorescence intensity and lifetime-based information simultaneously. Watch the full presentation here: https://microscopyfocus.com/confocal-microscopy-stellaris-showcase/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Tools & Techniques for the Study of Biologics & Antibody Drug Conjugates 47:57

cách đây 29 weeks trước47:57

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The fundamental cellular mechanisms of internalization and trafficking are crucial to many areas of cell biology, especially the proper function of therapeutic antibodies. Antibodies intended for use as antibody-drug conjugates (ADCs) should specifically bind to target cells and rapidly internalize via endocytosis. However, the ability to study these processes has historically been limited by the lack of tools that directly monitor the internalization and subsequent catabolism of extracellular material. In this episode, get a demo of rapid, high-throughput methods to screen for antibody internalization. You will also hear about techniques to directly visualize the internalization of therapeutic antibodies and ADCs in live cells for structure-activity relationship studies. You will also learn how the insights provided by these approaches have the potential to accelerate biotherapeutic lead generation and development. Watch the full presentation here: https://events.bitesizebio.com/tools-techniques-for-the-study-of/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Multiplex Annotated Tissue Imaging System (MANTIS®) 1:07:54

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Join us on Listen In as we unveil a breakthrough method for imaging skin immune cells, crucial for accurate diagnoses and personalized treatments. Traditional imaging faces challenges like high tissue autofluorescence and spectral spillover. However, our latest episode introduces a game-changing solution using the Leica SP8 microscope coupled with the MANTIS system. Learn how this innovative approach allows for the acquisition of up to 10 colors, providing detailed immune phenotyping of skin samples. Plus, discover how MANTIS simplifies complex imaging, making advanced analysis accessible and practical for routine clinical practice. Tune in to explore how this new technology can revolutionize the identification of skin immune cells and enhance diagnostic accuracy. Watch the full presentation here: https://microscopyfocus.com/multiplex-annotated-tissue-imaging/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Breaking Out of The Mold - A Panel Discussion of Alternative Careers 50:20

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Join us on Listen In for an inspiring episode that explores alternative careers for scientists who want to combine their artistic skills with their passion for science. If you love science but feel that traditional lab life isn’t for you, this episode is a must-listen. In this episode, we're joined by a panel of individuals who have carved out unique careers that merge science with creativity. Our panelists include a Marketing Communication Manager/Graphic Designer, an Artist in Residence, and a Science Rapper. These trailblazers will share their journeys, discuss how they found their unique paths, and provide insights into their innovative careers. Learn about the various opportunities available beyond the conventional academic and industry roles, and get inspired by the possibilities of combining science with art. Tune in to discover how you can turn your passion for science and art into a fulfilling career. Watch the full presentation here: https://events.bitesizebio.com/breaking-out-of-the-mold-a-panel-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Five-Color STED With a Single Depletion Laser and Fluorescence Lifetime Phasor Separation 56:33

cách đây 32 weeks trước56:33

56:33

Dive into the innovative world of STED microscopy and explore how researchers are pushing the boundaries of super-resolution imaging in this episode of Listen In. Traditional STED microscopy achieves high resolution by using a doughnut-shaped depletion laser to selectively suppress fluorescence, but multicolor experiments often face challenges such as laser misalignment and dye bleaching. Explore the game-changing technique of using a single depletion wavelength combined with phasor-based FLIM separation. This method allows scientists to distinguish multiple fluorophores with similar emission spectra but different fluorescent lifetimes, significantly increasing the number of usable spectral channels. Discover how researchers successfully depleted five fluorochromes across different channels using a single laser and utilized the phasor plot for quick and easy visualization of staining quality and lifetime analysis. This approach not only doubles the number of distinguishable colors in laser scanning microscopy but also simplifies the process, making it accessible to many scientists with minimal training. Watch the full presentation here: https://microscopyfocus.com/five-color-sted/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Grant Writing: Everything but the Science 32:47

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Dive into the often daunting world of grant applications. Securing funding is critical for any researcher, but the process can be filled with tricky tasks and complex requirements. Join us as we explore strategies to handle common challenges in grant applications with expert advice. Learn how to tackle issues like: • Career disruption • Reason for choosing your place of work • Track record relative to opportunity • Consumer involvement This episode guides you through the rationale behind these additional requirements, offers tips on how to effectively showcase your track record and collaborators, and provides insights on managing challenging grant situations. Don’t miss this essential advice that could make all the difference in securing your next grant! Watch the full presentation here: https://events.bitesizebio.com/grant-writing-everything-but-the-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com…

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Live-cell Fluorescence Lifetime Multiplexing Using Organic Fluorophores 45:33

cách đây 34 weeks trước45:33

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In this episode, we explore the cutting-edge techniques revolutionizing fluorescence microscopy. Traditional methods are limited to detecting three to four targets due to the spectral overlap of fluorophores. But now, scientists are breaking these barriers with fluorescence lifetime multiplexing. This innovative approach uses differences in fluorescence lifetimes to distinguish between similar dyes, allowing for more complex imaging. Hear how researchers combined lifetime multiplexing with spectral detection to analyze multiple targets simultaneously. Discover the exciting advancements made by characterizing 18 synthetic fluorophores and developing unique self-labeling protein tags. These breakthroughs enable the imaging of up to eight targets in four spectral channels, providing unprecedented detail in cellular studies. Watch the full presentation here: https://microscopyfocus.com/live-cell-fluorescence-lifetime-multiplexing-using-organic-fluorophores/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Stress in the Lab. Common Pitfalls and How to Avoid Them 49:19

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Stress is a universal challenge, and scientists face unique pressures such as impostor syndrome, the demand for positive results, and career uncertainty. These stressors can be overwhelming and often feel beyond our control. In this episode of Listen In , we explore the various stresses that scientists encounter in their daily lab life and discuss effective strategies to manage them. Learn how to prevent burnout, anxiety, and depression by addressing issues like perfectionism and impostor syndrome. Our expert shares personal insights and practical advice on cultivating a resilient and healthier growth mindset. Hit play to discover that you're not alone in feeling overwhelmed and that with the right mindset, you can navigate the pressures of a scientific career more effectively. Watch the full presentation here: https://events.bitesizebio.com/stress-in-the-lab-common-pitfalls-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Visualizing Protein-Protein Interactions by Non-fitting and Easy FRET-FLIM Approaches 1:10:53

cách đây 36 weeks trước1:10:53

1:10:53

Understanding molecular interactions in living cells is one of the key elements to deciphering the molecular mechanisms underpinning most cellular functions. Förster resonance energy transfer (FRET) is the gold standard for studying protein-protein interactions. Fluorescence lifetime imaging microscopy (FLIM) allows for a straightforward quantification of FRET based on the behavior of donor-only fluorescence. Join Dr. Padilla-Parra in this episode of Listen In to learn about some of the limitations of classical FRET approaches, how much information you can harness from FRET, and how lifetime-based non-fitting approaches such as minimal fraction of interacting donors (mFD) can provide a direct readout of protein-protein interactions in the cellular environment over time. Watch the full presentation here: https://microscopyfocus.com/visualizing-protein-protein-interactions-by-non-fitting-and-easy-fret-flim-approaches/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com…

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Optimizing Nuclei Extraction & Counting for Single Cell Sequencing 46:44

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Single cell sequencing samples must be prepared to the highest possible standard. Discover reliable techniques to prepare and validate nuclei samples for single cell sequencing. In this episode, learn how to perform essential quality control checks, including a quick and reliable method for quantifying nuclei, measuring extraction efficiency, and visualizing debris in a sample. You will also get tips to optimize your existing nuclei extraction protocols from an experienced Application Scientist. Watch the full presentation here: https://events.bitesizebio.com/optimizing-nuclei-extraction/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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How to Effectively Communicate Your Research 24:46

cách đây 38 weeks trước24:46

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Join Kate Christian as she demystifies the process of effectively communicating your research in the latest episode of Listen In . She describes the impact of research communication in and out of the lab and provides some guides to explaining your research in various contexts, such as lab tours, community visits, and other public engagements. As well as learning how to work out your h-index, you will learn how to describe the impact of your papers. Watch the full presentation here: https://events.bitesizebio.com/how-to-effectively-communicate-your-1/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Imaging GPCR Activity: Unravelling Mechanisms from Molecules to Morphology 1:08:22

cách đây 39 weeks trước1:08:22

1:08:22

Precise control of cellular communication and signaling is crucial for every physiological system, with G protein-coupled receptors (GPCRs, the largest family of signaling receptors) playing a central role as essential molecular sensors of a cell’s environment. Because of their diverse physiological and pathophysiological roles, GPCRs are also major drug targets. Our understanding of GPCR signaling has evolved from single receptors at the cell surface activating specific heterotrimeric G protein pathways, to an increasingly complex receptor signaling system. However, the complexity of GPCR signaling is translated to specific cellular and physiological responses is still unclear. In this episode, we explore some of the latest endeavors to uncover GPCR signaling mechanisms through a wide range of imaging applications, from analyzing single molecules to the identification of new subcellular signal platforms. Watch the full presentation here: https://microscopyfocus.com/imaging-gpcr-activity-unravelling-mechanisms-from-molecules-to-morphology/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Critical Analysis Of Research Papers: Practical Examples 42:45

cách đây 40 weeks trước42:45

42:45

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New TauSTED Tools for Gentle Live Imaging at Nanoscale Resolution 1:04:16

cách đây 41 weeks trước1:04:16

1:04:16

This episode of Listen In showcases the latest tools and developments for multiplex STED imaging of living specimens at nanoscale resolution. Confocal imaging is a fundamental tool for studying the complex interplay of biomolecules, molecular machines, and higher-order cellular structures owing to its optical sectioning, sensitivity, and temporal and spatial resolution capabilities. Imaging complex cellular structures at nanoscale resolution while characterizing the dynamics of multiple species in live specimens are emerging avenues to shed light on biological processes in their native physiological context. With the advent of STED, researchers have realized the visualization of intracellular structures at the nanoscale. Tune in to see how STED is enabling unprecedented insights into cellular behavior, interactions, and function. Watch the full presentation here: https://microscopyfocus.com/new-tausted-tools-for-gentle-live-imaging-at-nanoscale-resolution/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Finding The Ideal Hook for Your Science Story 37:25

cách đây 42 weeks trước37:25

37:25

Your research is important, but it can be hard to explain to others why it's important. In this episode of Listen In , you'll learn to find the ideal hook for your science story. This trick, borrowed from journalism, involves taking a step back and thinking about all the aspects of your research and where they might overlap with other topics that attract interest from other people. Knowing how your research connects with other areas is useful when writing grant proposals or giving public talks about your work because it helps you write or speak persuasively. Watch the full presentation here: https://events.bitesizebio.com/finding-the-ideal-hook-for-your/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Culture Media Preparation: Contamination Control and Retention of Critical Components 52:37

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Contamination poses a significant risk in cell culture, jeopardizing research outcomes and incurring costly setbacks. This episode of Listen In gives you valuable insights into often overlooked areas of your filtration processes and will help you safeguard your sensitive cell cultures. Explore the critical importance of sterile filtration in diverse applications leveraging Millipore® filtration technology. Hear about the alarming consequences of suboptimal filters in sensitive media composition, highlighting the financial and time investments at stake. Plus, discover how the use of Millipore® filters as the gold standard in both non-sterile and sterile filtration can mitigate contamination risks and uphold the integrity of your cultures. Our expert guest showcases the superior performance of Millipore® membranes and explains their advanced features that enable efficient particle retention and exceptional flow characteristics. Watch the full presentation here: https://events.bitesizebio.com/culture-media-preparation/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Automate Your Pipetting — Unleash Your Potential 40:49

cách đây 44 weeks trước40:49

40:49

Despite the growing number of new technologies and automated instruments, routine liquid handling is still a manual process in many labs. The trend towards automation is gaining importance, not only with complex applications, such as NGS library preparation but also with simple yet repetitive liquid handling steps where automated pipetting makes sense. A variety of automated liquid handling systems—suiting all budgets and workloads—are now available. These systems are designed to improve consistency and efficiency by eliminating both person-to-person and day-to-day variability. But how to get started with automation? When does it make sense to automate workflows and methods, and isn't it super complicated to implement an automated liquid handler in a lab? Get answers to these questions in this episode of Listen In ! Watch the full presentation here: https://events.bitesizebio.com/automate-your-pipetting-unleash/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Cellular Crosstalk in Neurodevelopmental Disorders 1:03:20

cách đây 45 weeks trước1:03:20

1:03:20

Inspired by mutations associated with neurodevelopmental disorders, Prof. Dr. Silvia Cappello’s group at the Ludwig Maximilian University of Munich aims to understand the role of extracellular mechanisms essential for the correct development of the human brain. Their research focuses on intrinsic and extrinsic mechanisms contributing to the formation of the brain by examining mutations in genes influencing cell-cell contacts, the extracellular matrix, and the secretion of vesicles. In this episode of Listen In , Silvia presents the latest research that makes an important contribution to our understanding of cell non-autonomous mechanisms in the development of neurodevelopmental disorders. She also explains how imaging workflows, using the Mica Imaging Microhub alongside confocal and super-resolution systems, support the group’s research. Watch the full presentation here: https://microscopyfocus.com/cellular-crosstalk-in-neurodevelopmental-disorders/ Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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End-point PCR Set-up and Optimization: Best Practice 57:59

cách đây 46 weeks trước57:59

57:59

In this episode of Listen In , master your PCR set-up for unparalleled results. Check out this enlightening discussion of the crucial factors that influence the outcome of PCR experiments and discover the parameters you can optimize for better and more consistent PCR results. You will get reliable advice directly from PCR experts at Eppendorf on key topics, including the core features of thermal cyclers and the importance of good pipetting techniques to minimize evaporation. This episode also features helpful insights into ideal reaction set-up, primer design, template DNA selection, and PCR optimization. Watch the full presentation here: https://events.bitesizebio.com/end-point-pcr-set-up-and/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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Approaches for More Transparent, Rigorous, and Reproducible Research 48:52

cách đây 47 weeks trước48:52

48:52

Improving open science and research reproducibility is both a technical and a social challenge. But how do we do it? One way is to design, conduct, and report research that can maximize the impact of our individual and collective investment in research. Plus, changing the research culture to prioritize 'getting it right' over 'getting it published' promotes transparency and reproducibility without losing the engine of innovation and discovery that drives scientific inquiry. In this episode of Listen In , get an overview of the fundamental challenges that reduce the credibility and reproducibility of research, how open science practices address these challenges, how you can incorporate them into your research, and how you can help promote a change in the research culture. Watch the full presentation here: https://events.bitesizebio.com/approaches-for-more-transparent/join Browse all episodes of the Listen In Series here: https://listen-in.bitesizebio.com/…

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New TauSTED Tools for Gentle Live Imaging at Nanoscale Resolution